Proglucagon: difference between revisions
Diff·revision 17 → 18·11:21, 2 Mar 2025
Difference between revision 17 and revision 18 of Proglucagon. 3 lines changed; the page grew by 727 bytes.
| Revision 17 — 13:24, 7 Feb 2025 Chromatokid (talk) expand §Gene and transcript 4,998 bytes ±0 | Revision 18 — 11:21, 2 Mar 2025 IpamorelinIsold (talk) split §Physiology from §Pharmacological exploitation 5,725 bytes +727 | ||
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| 40 | Immunoassays for the products of proglucagon are notoriously cross-reactive. Antisera raised against the C-terminus of glucagon detect glicentin and oxyntomodulin, both of which contain the glucagon sequence; assays raised against the N-terminus detect the major proglucagon fragment. Reported fasting glucagon concentrations vary several-fold between published methods.{{r|holst2007}} | 40 | Immunoassays for the products of proglucagon are notoriously cross-reactive. Antisera raised against the C-terminus of glucagon detect glicentin and oxyntomodulin, both of which contain the glucagon sequence; assays raised against the N-terminus detect the major proglucagon fragment. Reported fasting glucagon concentrations vary several-fold between published methods.{{r|holst2007}} |
| 41 | 41 | ||
| + | 42 | Sandwich assays using two antibodies against opposite ends of the mature glucagon sequence, and [[Liquid chromatography-mass spectrometry|LC-MS]] methods, have improved the position substantially.{{r|wewer2014}} Any comparison of glucagon data across studies should establish which assay was used before the numbers are treated as commensurable — this is a frequent source of apparent contradiction in the incretin literature.{{r|campbell2013}} | |
| + | 43 | ||
| 42 | == References == | 44 | == References == |
| 43 | {{reflist}} | 45 | {{reflist}} |
| ⋮ | ⋮ | ||
| 45 | <ref name="holst2007">Holst JJ. "The physiology of glucagon-like peptide 1." ''Physiological Reviews'' 87(4):1409–1439 (2007). PMID 17928588.</ref> | 47 | <ref name="holst2007">Holst JJ. "The physiology of glucagon-like peptide 1." ''Physiological Reviews'' 87(4):1409–1439 (2007). PMID 17928588.</ref> |
| 46 | <ref name="campbell2013">Campbell JE, Drucker DJ. "Pharmacology, physiology, and mechanisms of incretin hormone action." ''Cell Metabolism'' 17(6):819–837 (2013). PMID 23684623.</ref> | 48 | <ref name="campbell2013">Campbell JE, Drucker DJ. "Pharmacology, physiology, and mechanisms of incretin hormone action." ''Cell Metabolism'' 17(6):819–837 (2013). PMID 23684623.</ref> |
| + | 49 | <ref name="wewer2014">Wewer Albrechtsen NJ, Hartmann B, Veedfald S, et al. "Hyperglucagonaemia analysed by glucagon sandwich ELISA: nonspecific interference or truly elevated levels?" ''Diabetologia'' 57(9):1919–1926 (2014). DOI:10.1007/s00125-014-3283-z. PMID 24891019.</ref> | |
| 47 | 50 | ||
| 48 | == See also == | 51 | == See also == |