PeptidePedia The community reference

Reconstitution calculator (revision 7)

Old revision·06:22, 26 Sep 2024·Ref_Desk_Ron

This is an old revision of this page, as it stood at 06:22, 26 Sep 2024, saved by Ref_Desk_Ron with the summary replace bare ref with full citation. It may differ substantially from the current revision, and any error it contains may since have been corrected.
Reconstitution calculator
InputsVial mass (mg), diluent volume (mL), dose (mg or µg), syringe scale
OutputsConcentration, dose volume, syringe units, doses per vial
Reference tool infobox · conventions

The reconstitution calculator relates the four quantities involved in dissolving a lyophilised peptide and drawing a dose from it: the mass of solid in the vial, the volume of diluent added, the intended dose, and the reading on the syringe barrel. All four are connected by one division, and almost every error reported in the literature and in community accounts is a confusion between two of them rather than a failure of the arithmetic itself.[1]

The calculation is a routine unit conversion: concentration is mass divided by volume, dose volume is dose divided by concentration, and a syringe unit is one hundredth of a millilitre on the U-100 scale. The tool below performs those operations and shows each intermediate result, because the intermediate is where a mistake becomes visible.

The calculator

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Enter the label mass of the vial, the volume of diluent, and the intended dose. The tool reports the resulting concentration, the volume that dose occupies, the reading on the selected syringe scale, and how many doses the vial contains.

References

  1. ^ United States Pharmacopeia, General Chapter <797>, "Pharmaceutical Compounding — Sterile Preparations". USP–NF, current revision.