Mass spectrometry: difference between revisions
Diff·revision 6 → 7·00:26, 14 Aug 2024
Difference between revision 6 and revision 7 of Mass spectrometry. 4 lines changed; the page grew by 449 bytes.
| Revision 6 — 23:57, 7 Aug 2024 TFA_Counterion (talk) add the figure for the chromatogram and caption its axes 2,431 bytes +341 | Revision 7 — 00:26, 14 Aug 2024 CiteBot (talk) bot: sort category members 2,880 bytes +449 | ||
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| 10 | A purity determination by [[High-performance liquid chromatography|chromatography]] establishes that one species dominates a sample; it does not establish which species. These are separate questions and require separate determinations. A [[Certificate of analysis|certificate]] reporting purity but no identity leaves the more fundamental of the two unanswered.{{r|usp1503}} | 10 | A purity determination by [[High-performance liquid chromatography|chromatography]] establishes that one species dominates a sample; it does not establish which species. These are separate questions and require separate determinations. A [[Certificate of analysis|certificate]] reporting purity but no identity leaves the more fundamental of the two unanswered.{{r|usp1503}} |
| 11 | 11 | ||
| + | 12 | Peptides ionised by [[Electrospray ionisation|electrospray]] carry multiple charges, so a single peptide produces a series of peaks at different charge states. The instrument software deconvolves this envelope into one neutral mass, and it is that deconvolved mass that appears on a report.{{r|aebersold2003,usp736}} | |
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| 12 | == Ionisation and mass analysis == | 14 | == Ionisation and mass analysis == |
| 13 | Electrospray disperses a solution into charged droplets in an electric field; solvent evaporates and ions are released into the gas phase. It is a soft technique — the molecule survives largely intact — which is why it suits peptides and proteins. Matrix-assisted laser desorption is the other common soft method and tends to produce singly charged ions.{{r|aebersold2003}} | 15 | Electrospray disperses a solution into charged droplets in an electric field; solvent evaporates and ions are released into the gas phase. It is a soft technique — the molecule survives largely intact — which is why it suits peptides and proteins. Matrix-assisted laser desorption is the other common soft method and tends to produce singly charged ions.{{r|aebersold2003}} |
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| 21 | <ref name="aebersold2003">Aebersold R, Mann M. "Mass spectrometry-based proteomics." ''Nature'' 422(6928):198–207 (2003). DOI:10.1038/nature01511. PMID 12634793.</ref> | 23 | <ref name="aebersold2003">Aebersold R, Mann M. "Mass spectrometry-based proteomics." ''Nature'' 422(6928):198–207 (2003). DOI:10.1038/nature01511. PMID 12634793.</ref> |
| 22 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> | 24 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> |
| + | 25 | <ref name="usp736">United States Pharmacopeia, General Chapter <736>, ''Mass Spectrometry''.</ref> | |
| 23 | 26 | ||
| 24 | {{DEFAULTSORT:Mass spectrometry}} | 27 | {{DEFAULTSORT:Mass spectrometry}} |
| 25 | [[Category:Mass spectrometry]] | 28 | [[Category:Mass spectrometry]] |
| 26 | [[Category:Analytical methods]] | 29 | [[Category:Analytical methods]] |
| + | 30 | [[Category:Analytical science]] | |
| 27 | 31 |