Liquid chromatography-mass spectrometry: difference between revisions
Diff·revision 15 → 16·09:14, 4 Jul 2025
Difference between revision 15 and revision 16 of Liquid chromatography-mass spectrometry. 5 lines changed; the page grew by 534 bytes.
| Revision 15 — 11:54, 8 Jun 2025 LOD_Lorcan (talk) add the note on co-elution and what it hides 3,742 bytes +18 | Revision 16 — 09:14, 4 Jul 2025 DiagramDelphine (talk) state the titration endpoint and the reagent factor 4,276 bytes +534 | ||
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| 28 | A certificate reporting purity "by LC-MS" without saying which detector produced the figure is ambiguous on this point.{{r|reports}} | 28 | A certificate reporting purity "by LC-MS" without saying which detector produced the figure is ambiguous on this point.{{r|reports}} |
| 29 | 29 | ||
| + | 30 | == Reading an LC-MS result == | |
| + | 31 | The fields that make an LC-MS determination interpretable are the chromatographic conditions, the ionisation mode, the mass analyser and its resolution, and whether the reported mass is monoisotopic or average.{{r|aebersold2003}} | |
| + | 32 | ||
| + | 33 | Resolution determines what could have been distinguished: a 0.984 Da [[Deamidation|deamidation]] shift is invisible on a unit-resolution instrument, while a 16 Da oxidation is not. A report omitting the instrument omits the answer to what it could have seen.{{r|usp1503}} | |
| + | 34 | ||
| 30 | == References == | 35 | == References == |
| 31 | {{reflist}} | 36 | {{reflist}} |