High-performance liquid chromatography: difference between revisions
Diff·revision 26 → 27·14:43, 4 Oct 2025
Difference between revision 26 and revision 27 of High-performance liquid chromatography. 2 lines changed; the page grew by 416 bytes.
| Revision 26 — 13:12, 13 Sep 2025 HyphenHarriet (talk) space between number and unit per PP:MOS 6,815 bytes ±0 | Revision 27 — 14:43, 4 Oct 2025 ChromCleanupCass (talk) ce 7,231 bytes +416 | ||
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| 52 | Scaling the same separation up, with wider columns and higher loadings, gives [[Preparative HPLC purification|preparative HPLC]], which is a purification step rather than an analytical one. The chemistry is the same; the objective is collecting a fraction rather than measuring one. | 52 | Scaling the same separation up, with wider columns and higher loadings, gives [[Preparative HPLC purification|preparative HPLC]], which is a purification step rather than an analytical one. The chemistry is the same; the objective is collecting a fraction rather than measuring one. |
| 53 | 53 | ||
| + | 54 | The relationship between the two matters for interpreting a certificate. Material purified by preparative chromatography and then analysed by an analytical method derived from the same separation will look clean, because impurities that co-eluted during purification will co-elute during analysis. An orthogonal method — different column chemistry or different mode — is what tests that assumption.{{r|usp621}} | |
| + | 55 | ||
| 54 | == References == | 56 | == References == |
| 55 | {{reflist}} | 57 | {{reflist}} |