Electrospray ionisation: difference between revisions
Diff·revision 5 → 6·20:53, 2 Nov 2024
Difference between revision 5 and revision 6 of Electrospray ionisation. 3 lines changed; the page grew by 382 bytes.
| Revision 5 — 16:16, 20 Oct 2024 KarlFischerKit (talk) add the detection wavelength 2,185 bytes +206 | Revision 6 — 20:53, 2 Nov 2024 AAA_Analyst (talk) add the plate-count expression and define its terms 2,567 bytes +382 | ||
|---|---|---|---|
| 18 | The charge states observed depend on the number of ionisable sites and on solution conditions. Acidic mobile phases favour protonation and positive-mode operation, which is why peptide LC-MS is usually run in positive mode with an acidic modifier.{{r|aebersold2003}} | 18 | The charge states observed depend on the number of ionisable sites and on solution conditions. Acidic mobile phases favour protonation and positive-mode operation, which is why peptide LC-MS is usually run in positive mode with an acidic modifier.{{r|aebersold2003}} |
| 19 | 19 | ||
| + | 20 | Ion suppression is the characteristic difficulty: co-eluting species compete for charge, so a compound's signal depends on what else is eluting at the same time. This is why the technique is not quantitative without calibration.{{r|usp1503}} | |
| + | 21 | ||
| 20 | == References == | 22 | == References == |
| 21 | {{reflist}} | 23 | {{reflist}} |
| 22 | <ref name="fenn1989">Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. "Electrospray ionization for mass spectrometry of large biomolecules." ''Science'' 246(4926):64–71 (1989). PMID 2675315.</ref> | 24 | <ref name="fenn1989">Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. "Electrospray ionization for mass spectrometry of large biomolecules." ''Science'' 246(4926):64–71 (1989). PMID 2675315.</ref> |
| 23 | <ref name="aebersold2003">Aebersold R, Mann M. "Mass spectrometry-based proteomics." ''Nature'' 422(6928):198–207 (2003). PMID 12634793.</ref> | 25 | <ref name="aebersold2003">Aebersold R, Mann M. "Mass spectrometry-based proteomics." ''Nature'' 422(6928):198–207 (2003). PMID 12634793.</ref> |
| + | 26 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> | |
| 24 | 27 | ||
| 25 | {{DEFAULTSORT:Electrospray ionisation}} | 28 | {{DEFAULTSORT:Electrospray ionisation}} |