Crude peptide: difference between revisions
Diff·revision 5 → 6·06:48, 30 Oct 2024
Difference between revision 5 and revision 6 of Crude peptide. 3 lines changed; the page grew by 347 bytes.
| Revision 5 — 16:31, 12 Oct 2024 CrudePeptidePearl (talk) attribute the stability figure to the study and its duration 2,285 bytes +415 | Revision 6 — 06:48, 30 Oct 2024 IpamorelinIsold (talk) expand §What is in it 2,632 bytes +347 | ||
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| 17 | Cleavage adds its own. Strongly acidic conditions release reactive cations from the protecting groups, which can alkylate tryptophan, methionine and cysteine unless scavengers capture them; oxidation and, for susceptible sequences, aspartimide formation also occur. | 17 | Cleavage adds its own. Strongly acidic conditions release reactive cations from the protecting groups, which can alkylate tryptophan, methionine and cysteine unless scavengers capture them; oxidation and, for susceptible sequences, aspartimide formation also occur. |
| 18 | 18 | ||
| + | 19 | Non-peptide material is present too: scavengers, residual cleavage reagent, and trifluoroacetate. A crude figure quoted as a percentage is normally a chromatographic [[Area percent purity|area percent]], so it says nothing about how much of the solid is peptide at all. See [[Peptide content]].{{r|usp1503}} | |
| + | 20 | ||
| 19 | == References == | 21 | == References == |
| 20 | {{reflist}} | 22 | {{reflist}} |
| ⋮ | ⋮ | ||
| 26 | [[Category:Peptide synthesis]] | 28 | [[Category:Peptide synthesis]] |
| 27 | [[Category:Purification]] | 29 | [[Category:Purification]] |
| + | 30 | [[Category:Manufacturing and supply]] | |
| 28 | 31 |