Solid-phase peptide synthesis (revision 1)
Old revision·06:30, 25 Jun 2024·SupplyWatchSuri
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| Solid-phase peptide synthesis | |
|---|---|
| Abbreviation | SPPS |
| Introduced | 1963 |
| Support | Polystyrene or polyethylene glycol resin |
| Dominant chemistry | Fmoc |
| Analytical method infobox · conventions | |
Solid-phase peptide synthesis (SPPS) assembles a peptide on an insoluble polymeric support. Because the growing chain is anchored, excess reagents and soluble by-products are removed simply by washing, which is what makes stepwise synthesis of long sequences practical.[1]
The cycle is: remove the temporary N-terminal protecting group, wash, couple the next protected amino acid in excess, wash. Excess reagent drives each coupling towards completion, and the washing that removes it is the operation solution-phase synthesis cannot perform cheaply.[2]
References
- ^ Merrifield RB. "Solid phase peptide synthesis. I. The synthesis of a tetrapeptide." Journal of the American Chemical Society 85(14):2149–2154 (1963). DOI:10.1021/ja00897a025.
- ^ Behrendt R, White P, Offer J. "Advances in Fmoc solid-phase peptide synthesis." Journal of Peptide Science 22(1):4–27 (2016). PMID 26785684.
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