Solid-phase peptide synthesis: difference between revisions
Diff·revision 6 → 7·01:38, 13 Aug 2024
Difference between revision 6 and revision 7 of Solid-phase peptide synthesis. 2 lines changed; the page grew by 324 bytes.
| Revision 6 — 19:37, 31 Jul 2024 SemaglutideSasha (talk) rm the vendor-specific packaging detail; not general enough for the article 2,254 bytes +361 | Revision 7 — 01:38, 13 Aug 2024 RepackRadek (talk) correct the freezer temperature range given for long-term storage 2,578 bytes +324 | ||
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| 11 | The cycle is: remove the temporary N-terminal protecting group, wash, couple the next protected amino acid in excess, wash. Excess reagent drives each coupling towards completion, and the washing that removes it is the operation solution-phase synthesis cannot perform cheaply.{{r|behrendt2016}} | 11 | The cycle is: remove the temporary N-terminal protecting group, wash, couple the next protected amino acid in excess, wash. Excess reagent drives each coupling towards completion, and the washing that removes it is the operation solution-phase synthesis cannot perform cheaply.{{r|behrendt2016}} |
| 12 | 12 | ||
| + | 13 | Its principal limitation follows from the same design. Intermediates cannot be purified — everything anchored to the resin travels together — so a failed coupling generates a deletion sequence that persists to the end and must be separated at the [[Preparative HPLC purification|purification]] stage.{{r|behrendt2016}} | |
| + | 14 | ||
| 13 | == Support and linker == | 15 | == Support and linker == |
| 14 | The resin is typically cross-linked polystyrene or a polyethylene glycol–polystyrene composite, functionalised with a linker that attaches the first residue and defines how the finished peptide is released.{{r|behrendt2016}} | 16 | The resin is typically cross-linked polystyrene or a polyethylene glycol–polystyrene composite, functionalised with a linker that attaches the first residue and defines how the finished peptide is released.{{r|behrendt2016}} |