Solid-phase peptide synthesis: difference between revisions
Diff·revision 13 → 14·19:30, 15 Nov 2024
Difference between revision 13 and revision 14 of Solid-phase peptide synthesis. 3 lines changed; the page grew by 484 bytes.
| Revision 13 — 22:48, 26 Oct 2024 CDMO_Caradoc (talk) split §Cold chain from §Storage after receipt 3,377 bytes +28 | Revision 14 — 19:30, 15 Nov 2024 PreregPriya (talk) expand §Difficult sequences 3,861 bytes +484 | ||
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| 34 | | Lower resin loading | Reduces chain crowding | | 34 | | Lower resin loading | Reduces chain crowding | |
| 35 | 35 | ||
| + | 36 | These are process choices rather than quality attributes, and they do not appear on a [[Certificate of analysis|certificate]]. Their consequences do: a sequence handled poorly gives a crude with a characteristic deletion at the difficult position, which a sufficiently specific method will resolve as a distinct related substance.{{r|usp1503}} | |
| + | 37 | ||
| 36 | == References == | 38 | == References == |
| 37 | {{reflist}} | 39 | {{reflist}} |
| 38 | <ref name="merrifield1963">Merrifield RB. "Solid phase peptide synthesis. I. The synthesis of a tetrapeptide." ''Journal of the American Chemical Society'' 85(14):2149–2154 (1963). DOI:10.1021/ja00897a025.</ref> | 40 | <ref name="merrifield1963">Merrifield RB. "Solid phase peptide synthesis. I. The synthesis of a tetrapeptide." ''Journal of the American Chemical Society'' 85(14):2149–2154 (1963). DOI:10.1021/ja00897a025.</ref> |
| 39 | <ref name="behrendt2016">Behrendt R, White P, Offer J. "Advances in Fmoc solid-phase peptide synthesis." ''Journal of Peptide Science'' 22(1):4–27 (2016). PMID 26785684.</ref> | 41 | <ref name="behrendt2016">Behrendt R, White P, Offer J. "Advances in Fmoc solid-phase peptide synthesis." ''Journal of Peptide Science'' 22(1):4–27 (2016). PMID 26785684.</ref> |
| + | 42 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> | |
| 40 | 43 | ||
| 41 | {{DEFAULTSORT:Solid-phase peptide synthesis}} | 44 | {{DEFAULTSORT:Solid-phase peptide synthesis}} |