Reverse-phase HPLC: difference between revisions
Diff·revision 6 → 7·04:41, 2 Sep 2024
Difference between revision 6 and revision 7 of Reverse-phase HPLC. 3 lines changed; the page grew by 268 bytes.
| Revision 6 — 14:36, 12 Aug 2024 TFA_Counterion (talk) add short description 2,744 bytes +439 | Revision 7 — 04:41, 2 Sep 2024 TirzTaxonomist (talk) space between number and unit per PP:MOS 3,012 bytes +268 | ||
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| 26 | Gradient duration is the parameter with the largest effect on a reported purity figure. A shallow gradient run over an hour resolves species a fifteen-minute gradient merges, and the merged determination returns a higher number on identical material. Neither is wrong; they are different measurements. See [[Purity claim inflation]].{{r|mant1996}} | 26 | Gradient duration is the parameter with the largest effect on a reported purity figure. A shallow gradient run over an hour resolves species a fifteen-minute gradient merges, and the merged determination returns a higher number on identical material. Neither is wrong; they are different measurements. See [[Purity claim inflation]].{{r|mant1996}} |
| 27 | 27 | ||
| + | 28 | Temperature is the parameter most often omitted from certificates. Elevated column temperature sharpens peaks for large peptides by reducing conformational heterogeneity, and a method developed at 60 °C will not reproduce at ambient. | |
| + | 29 | ||
| 28 | == References == | 30 | == References == |
| 29 | {{reflist}} | 31 | {{reflist}} |
| ⋮ | ⋮ | ||
| 35 | [[Category:Chromatography]] | 37 | [[Category:Chromatography]] |
| 36 | [[Category:Analytical methods]] | 38 | [[Category:Analytical methods]] |
| + | 39 | [[Category:Analytical science]] | |
| 37 | 40 |