Retention time (revision 1)
Old revision·09:00, 15 Aug 2024·TrialsTabitha
This is an old revision of this page, as it stood at 09:00, 15 Aug 2024, saved by TrialsTabitha with the summary new article: analytical method, expansion welcome. It may differ substantially from the current revision, and any error it contains may since have been corrected.
| Retention time | |
|---|---|
| Symbol | t_R |
| Depends on | Column, mobile phase, gradient, flow, temperature |
| Relative measure | Relative retention time, against a reference peak |
| Topic infobox · conventions | |
Retention time is the interval between injection and the appearance of a component's peak maximum at the detector. Under fixed conditions it is characteristic of a substance and reproducible, which makes it the basis of chromatographic identification.[1]
It is not a property of the substance alone. Column chemistry and dimensions, mobile-phase composition, gradient profile, flow rate, temperature and even instrument dwell volume all change it, so a retention time is only meaningful alongside the conditions that produced it.[1]
References
Categories: