Resin cleavage (revision 11)
Old revision·15:39, 29 Jan 2025·PeakShapePol
| Resin cleavagePeptide synthesis | |
|---|---|
| Reagent, Fmoc chemistry | Trifluoroacetic acid with scavengers |
| Simultaneous | Side-chain deprotection |
| Isolation | Precipitation into cold ether |
| Analytical method infobox · conventions | |
Resin cleavage is the step that releases an assembled peptide from the solid support. In Fmoc chemistry it also removes the acid-labile side-chain protecting groups, so a single treatment with concentrated trifluoroacetic acid accomplishes both.[1]
The removed protecting groups generate reactive carbocations, which will alkylate electron-rich side chains — tryptophan, methionine, cysteine and tyrosine — unless captured. Scavengers are included in the cleavage cocktail for that purpose, and their selection depends on which residues the sequence contains.[1]
Cleavage is therefore not a neutral release step. It is a chemical operation with its own characteristic impurities, and a substantial part of the difference between a clean and a dirty crude is decided here.[2]
Cocktails and scavengers
[edit]A typical cocktail is trifluoroacetic acid with a few per cent each of water, triisopropylsilane and, where cysteine or methionine is present, a thiol scavenger. Water and silane trap the tert-butyl and trityl cations; thiols address the more persistent species.[1]
Time and temperature are the other variables. Arginine's sulfonyl protecting groups are the slowest to remove, so a peptide rich in arginine requires longer treatment — during which more sensitive residues are exposed to acid for longer.
The trade-off is direct: insufficient time leaves protected species in the crude, excessive time generates degradation. This is one of the process choices that a certificate does not report but whose consequences it may show.[2]
Isolation
[edit]After cleavage the peptide is precipitated by adding the acidic solution to a large volume of cold diethyl ether, in which peptides are insoluble and the scavengers and cleaved protecting groups are soluble. The precipitate is collected, washed with further cold ether, and dried.[1]
Precipitation is a crude purification and removes small-molecule material efficiently while doing nothing about sequence-related impurities, which remain and are the business of preparative chromatography.
Residual ether is a residual solvent of Class 3, and residual trifluoroacetic acid becomes the counterion of the isolated solid.[2]