Preparative HPLC purification: difference between revisions
Diff·revision 6 → 7·18:47, 3 Oct 2024
Difference between revision 6 and revision 7 of Preparative HPLC purification. 3 lines changed; the page grew by 388 bytes.
| Revision 6 — 10:46, 19 Sep 2024 ThymosinTamsin (talk) fix a broken wikilink 2,308 bytes +38 | Revision 7 — 18:47, 3 Oct 2024 StubSorterBot (talk) bot: normalise unit spacing 2,696 bytes +388 | ||
|---|---|---|---|
| 17 | Loading is limited by overload. As the mass injected rises, peaks broaden and distort and resolution falls, so the practical loading is set by how much distortion the required purity tolerates. Touching-band operation, in which adjacent peaks just meet, is the usual economic optimum. | 17 | Loading is limited by overload. As the mass injected rises, peaks broaden and distort and resolution falls, so the practical loading is set by how much distortion the required purity tolerates. Touching-band operation, in which adjacent peaks just meet, is the usual economic optimum. |
| 18 | 18 | ||
| + | 19 | Fraction collection may be time-based or triggered by detector signal. Detector signals saturate at preparative loadings, so collection windows are often set by prior experiment rather than by real-time response, and a shift in retention between runs then costs yield or purity.{{r|usp621}} | |
| + | 20 | ||
| 19 | == References == | 21 | == References == |
| 20 | {{reflist}} | 22 | {{reflist}} |
| 21 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> | 23 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> |
| 22 | <ref name="mant1996">Mant CT, Hodges RS. "Analysis of peptides by high-performance liquid chromatography." ''Methods in Enzymology'' 271:3–50 (1996). PMID 8782429.</ref> | 24 | <ref name="mant1996">Mant CT, Hodges RS. "Analysis of peptides by high-performance liquid chromatography." ''Methods in Enzymology'' 271:3–50 (1996). PMID 8782429.</ref> |
| + | 25 | <ref name="usp621">United States Pharmacopeia, General Chapter <621>, ''Chromatography''.</ref> | |
| 23 | 26 | ||
| 24 | {{DEFAULTSORT:Preparative HPLC purification}} | 27 | {{DEFAULTSORT:Preparative HPLC purification}} |