Preparative HPLC purification: difference between revisions
Diff·revision 19 → 20·19:16, 3 Jul 2025
Difference between revision 19 and revision 20 of Preparative HPLC purification. 2 lines changed; the page grew by 284 bytes.
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| 33 | This is not a criticism of any particular practice — using the same chemistry is the obvious first choice — but it means that a purity figure from a method related to the purification is a weaker claim than one from an orthogonal method. Orthogonality can come from a different stationary-phase chemistry, a different modifier, a different pH, or a different mode altogether such as ion-exchange. | 33 | This is not a criticism of any particular practice — using the same chemistry is the obvious first choice — but it means that a purity figure from a method related to the purification is a weaker claim than one from an orthogonal method. Orthogonality can come from a different stationary-phase chemistry, a different modifier, a different pH, or a different mode altogether such as ion-exchange. |
| 34 | 34 | ||
| + | 35 | The point generalises: a determination is informative in proportion to the ways it could have failed. A method that could not have detected a particular impurity provides no evidence about that impurity, however precise its number. See [[Analytical method validation]].{{r|usp1503}} | |
| + | 36 | ||
| 35 | == References == | 37 | == References == |
| 36 | {{reflist}} | 38 | {{reflist}} |