Peptide synthesis: difference between revisions
Diff·revision 4 → 5·23:39, 24 Jul 2024
Difference between revision 4 and revision 5 of Peptide synthesis. 2 lines changed; the page grew by 311 bytes.
| Revision 4 — 22:25, 13 Jul 2024 StateBoardStace (talk) copyedit 2,036 bytes ±0 | Revision 5 — 23:39, 24 Jul 2024 Chromatokid (talk) add the shelf temperature and the primary drying step to the cycle description 2,347 bytes +311 | ||
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| 15 | Protecting groups are what make selectivity possible. The temporary N-terminal group is removed once per cycle; side-chain groups are orthogonal to it and survive until final cleavage. The choice of scheme — Fmoc with acid-labile side chains, or Boc with more forcing conditions — defines the whole chemistry that follows. See [[Fmoc chemistry]]. | 15 | Protecting groups are what make selectivity possible. The temporary N-terminal group is removed once per cycle; side-chain groups are orthogonal to it and survive until final cleavage. The choice of scheme — Fmoc with acid-labile side chains, or Boc with more forcing conditions — defines the whole chemistry that follows. See [[Fmoc chemistry]]. |
| 16 | 16 | ||
| + | 17 | Coupling is driven by an activating reagent that converts the carboxyl group into a reactive species. Reagent choice affects both speed and the degree of racemisation at the activated centre, and is one of the main levers in optimising a difficult sequence. See [[Peptide coupling reagent]].{{r|behrendt2016}} | |
| + | 18 | ||
| 17 | == References == | 19 | == References == |
| 18 | {{reflist}} | 20 | {{reflist}} |