Peptide aggregation: difference between revisions
Diff·revision 5 → 6·18:23, 14 Nov 2024
Difference between revision 5 and revision 6 of Peptide aggregation. 2 lines changed; the page grew by 356 bytes.
| Revision 5 — 18:42, 5 Nov 2024 Retention_Time_Rae (talk) give the limit of quantitation with the signal-to-noise ratio it assumes 2,687 bytes +446 | Revision 6 — 18:23, 14 Nov 2024 TFA_Counterion (talk) clarify that area percent is not mass percent 3,043 bytes +356 | ||
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| 10 | The distinction between the two is central and is frequently lost. [[Liraglutide|Liraglutide]] self-associates into heptamers in its formulation, and that association contributes to its duration of action; [[Amylin|human amylin]] forms fibrils, which is why it could not be developed as a medicine without engineering. Both are "aggregation" in a loose sense and they are not the same phenomenon.{{r|westermark2011}} | 10 | The distinction between the two is central and is frequently lost. [[Liraglutide|Liraglutide]] self-associates into heptamers in its formulation, and that association contributes to its duration of action; [[Amylin|human amylin]] forms fibrils, which is why it could not be developed as a medicine without engineering. Both are "aggregation" in a loose sense and they are not the same phenomenon.{{r|westermark2011}} |
| 11 | 11 | ||
| + | 12 | Aggregation is driven by concentration, by pH near the isoelectric point, by hydrophobic surface exposure, by agitation at air–liquid interfaces, and by temperature. It is the degradation route most likely to be visible: a solution that has gone faintly hazy after [[Reconstitution of lyophilised peptides|reconstitution]] has changed.{{r|manning2010}} | |
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| 12 | == Mechanisms == | 14 | == Mechanisms == |
| 13 | Aggregation proceeds through partially unfolded or conformationally exposed states rather than from the native state directly. Anything that increases the population of such states — heat, interfaces, shear, extremes of pH — increases the rate.{{r|manning2010}} | 15 | Aggregation proceeds through partially unfolded or conformationally exposed states rather than from the native state directly. Anything that increases the population of such states — heat, interfaces, shear, extremes of pH — increases the rate.{{r|manning2010}} |