Peptide aggregation: difference between revisions
Diff·revision 22 → 23·16:01, 26 Aug 2025
Difference between revision 22 and revision 23 of Peptide aggregation. 2 lines changed; the page grew by 322 bytes.
| Revision 22 — 04:22, 5 Aug 2025 EscalationEira (talk) clarify that mass spectrometry confirms identity, not purity 5,194 bytes ±0 | Revision 23 — 16:01, 26 Aug 2025 IsocraticIla (talk) add the ionisation mode to the mass-spectrometry description 5,516 bytes +322 | ||
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| 38 | Handling practices that reduce it are well established: dissolve by adding diluent down the vial wall rather than directly onto the cake, swirl rather than shake, avoid repeated freeze–thaw, and keep solutions cold. These follow from the mechanisms rather than from any particular guidance document. See [[Reconstitution of lyophilised peptides]]. | 38 | Handling practices that reduce it are well established: dissolve by adding diluent down the vial wall rather than directly onto the cake, swirl rather than shake, avoid repeated freeze–thaw, and keep solutions cold. These follow from the mechanisms rather than from any particular guidance document. See [[Reconstitution of lyophilised peptides]]. |
| 39 | 39 | ||
| + | 40 | For certificates, the point is one of method coverage. A specification for an aggregation-prone peptide that reports only reverse-phase purity omits the attribute most likely to fail for that chemistry. That is a statement about which questions the document answers, not about the material it describes.{{r|manning2010}} | |
| + | 41 | ||
| 40 | == References == | 42 | == References == |
| 41 | {{reflist}} | 43 | {{reflist}} |