Peptide aggregation: difference between revisions
Diff·revision 14 → 15·15:17, 22 Mar 2025
Difference between revision 14 and revision 15 of Peptide aggregation. 2 lines changed; the page grew by 336 bytes.
| Revision 14 — 16:06, 10 Mar 2025 Chromatokid (talk) add the note on co-elution and what it hides 4,041 bytes +13 | Revision 15 — 15:17, 22 Mar 2025 DiluentDiya (talk) correct the resolution formula — the denominator is the sum of the widths 4,377 bytes +336 | ||
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| 31 | The last row is the important one for reading certificates. A reverse-phase purity determination is run under denaturing conditions with organic solvent and acid; aggregates dissociate and are counted as monomer. A high purity figure by that method says nothing about aggregation state.{{r|usp1503}} | 31 | The last row is the important one for reading certificates. A reverse-phase purity determination is run under denaturing conditions with organic solvent and acid; aggregates dissociate and are counted as monomer. A high purity figure by that method says nothing about aggregation state.{{r|usp1503}} |
| 32 | 32 | ||
| + | 33 | Size-exclusion chromatography is the ordinary determination for aggregate content, and its result depends on the mobile phase: a dissociating buffer will report a cleaner profile than a native one on the same material. For an amylin analogue or an acylated incretin peptide, which one was used is a material question.{{r|manning2010}} | |
| + | 34 | ||
| 33 | == References == | 35 | == References == |
| 34 | {{reflist}} | 36 | {{reflist}} |