Lyophilisation: difference between revisions
Diff·revision 24 → 25·10:54, 22 Dec 2024
Difference between revision 24 and revision 25 of Lyophilisation. 1 line changed; the page grew by 188 bytes.
| Revision 24 — 03:44, 14 Dec 2024 CategoryBot (talk) bot: normalise citation format 12,142 bytes +1,276 | Revision 25 — 10:54, 22 Dec 2024 FlowTrialFraser (talk) move unsourced paragraph to talk 12,330 bytes +188 | ||
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| 13 | | Shelf temperature, secondary drying = 20–40 °C | 13 | | Shelf temperature, secondary drying = 20–40 °C |
| 14 | }} | 14 | }} |
| + | 15 | {{hatnote|For the reverse operation performed by the end user, see [[Reconstitution of lyophilised peptides]]. For measurement of the water that remains, see [[Karl Fischer titration]].}} | |
| 15 | 16 | ||
| 16 | '''Lyophilisation''', also called '''freeze-drying''', is a dehydration process in which a solution is frozen and the ice is then removed by sublimation at a pressure below the triple point of water, leaving a porous solid whose volume approximates that of the original fill. It is the standard final step in the isolation of synthetic [[peptide|peptides]] after [[Preparative HPLC purification|preparative purification]], and the reason a research peptide is distributed as a dry cake in a sealed [[vial]] rather than as a solution.{{r|nail2002}} | 17 | '''Lyophilisation''', also called '''freeze-drying''', is a dehydration process in which a solution is frozen and the ice is then removed by sublimation at a pressure below the triple point of water, leaving a porous solid whose volume approximates that of the original fill. It is the standard final step in the isolation of synthetic [[peptide|peptides]] after [[Preparative HPLC purification|preparative purification]], and the reason a research peptide is distributed as a dry cake in a sealed [[vial]] rather than as a solution.{{r|nail2002}} |