Liquid chromatography-mass spectrometry: difference between revisions
Diff·revision 11 → 12·23:15, 26 Mar 2025
Difference between revision 11 and revision 12 of Liquid chromatography-mass spectrometry. 3 lines changed; the page grew by 273 bytes.
| Revision 11 — 16:13, 24 Feb 2025 CiteBot (talk) bot: flag bare reference 3,451 bytes ±0 | Revision 12 — 23:15, 26 Mar 2025 LabRangeLindy (talk) add the reference standard and its traceability 3,724 bytes +273 | ||
|---|---|---|---|
| 26 | This is why a purity figure is normally reported from ultraviolet detection rather than from mass detection: ultraviolet response at 214 nm is roughly proportional to the number of peptide bonds and is therefore a reasonable proxy for mass across related peptides, whereas mass-spectrometric response is not.{{r|usp1503}} | 26 | This is why a purity figure is normally reported from ultraviolet detection rather than from mass detection: ultraviolet response at 214 nm is roughly proportional to the number of peptide bonds and is therefore a reasonable proxy for mass across related peptides, whereas mass-spectrometric response is not.{{r|usp1503}} |
| 27 | 27 | ||
| + | 28 | A certificate reporting purity "by LC-MS" without saying which detector produced the figure is ambiguous on this point.{{r|reports}} | |
| + | 29 | ||
| 28 | == References == | 30 | == References == |
| 29 | {{reflist}} | 31 | {{reflist}} |
| ⋮ | ⋮ | ||
| 31 | <ref name="fenn1989">Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. "Electrospray ionization for mass spectrometry of large biomolecules." ''Science'' 246(4926):64–71 (1989). PMID 2675315.</ref> | 33 | <ref name="fenn1989">Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. "Electrospray ionization for mass spectrometry of large biomolecules." ''Science'' 246(4926):64–71 (1989). PMID 2675315.</ref> |
| 32 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> | 34 | <ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref> |
| + | 35 | <ref name="reports">PeptidePedia Wiki community test-report tally, 2024–2026 (self-reported; see [[Project:Sourcing_guidelines]]).</ref> | |
| 33 | 36 | ||
| 34 | == See also == | 37 | == See also == |