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Limit of detection: difference between revisions

Diff·revision 3 → 4·13:36, 14 Sep 2024

Difference between revision 3 and revision 4 of Limit of detection. 6 lines changed; the page grew by 815 bytes.

Revision 3 — 21:31, 27 Aug 2024
NewLabNell (talk)
clarify the difference between related substances and total impurities
1,742 bytes ±0
Revision 4 — 13:36, 14 Sep 2024
CRP_Cormac (talk)
rm the claim that the method is stability-indicating without a forced-degradation study
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10The distinction is practical rather than pedantic. Between the two limits an analyte is detectable but not measurable, and a determination reported in that region carries an uncertainty comparable with the value itself.{{r|ich_q2}}10The distinction is practical rather than pedantic. Between the two limits an analyte is detectable but not measurable, and a determination reported in that region carries an uncertainty comparable with the value itself.{{r|ich_q2}}
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+12For peptide impurity work the relevant question is usually not what the limit is in absolute terms but whether it is below the reporting threshold. An impurity present at 0.05% is irrelevant if the method cannot see below 0.5%, and a certificate reporting "no impurities detected" is a statement about the method as much as about the material.{{r|usp1503}}
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12== How it is determined ==14== How it is determined ==
13Three approaches are recognised. The signal-to-noise approach compares the analyte signal with baseline noise and takes a ratio of about 3:1 for detection and 10:1 for quantitation; it is simple and is the usual choice for chromatographic methods. The standard-deviation-of-the-blank approach uses replicate blank measurements. The calibration-curve approach uses the residual standard deviation of the regression and its slope.{{r|ich_q2}}15Three approaches are recognised. The signal-to-noise approach compares the analyte signal with baseline noise and takes a ratio of about 3:1 for detection and 10:1 for quantitation; it is simple and is the usual choice for chromatographic methods. The standard-deviation-of-the-blank approach uses replicate blank measurements. The calibration-curve approach uses the residual standard deviation of the regression and its slope.{{r|ich_q2}}
15The three do not necessarily agree, and a reported limit should state which was used. Signal-to-noise figures in particular depend on how noise was measured — over what region of the baseline, and after what smoothing — and are not comparable between laboratories without that detail.17The three do not necessarily agree, and a reported limit should state which was used. Signal-to-noise figures in particular depend on how noise was measured — over what region of the baseline, and after what smoothing — and are not comparable between laboratories without that detail.
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+19The limit is a property of the method as applied, not of the technique. The same instrument with a longer injection, a more concentrated preparation or a more sensitive detector setting has a different limit.{{r|usp621}}
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17== References ==21== References ==
18{{reflist}}22{{reflist}}
19<ref name="ich_q2">International Council for Harmonisation, ''Q2(R2): Validation of Analytical Procedures'' (2023).</ref>23<ref name="ich_q2">International Council for Harmonisation, ''Q2(R2): Validation of Analytical Procedures'' (2023).</ref>
+24<ref name="usp1503">United States Pharmacopeia, General Chapter <1503>, ''Quality Attributes of Synthetic Peptide Drug Substances''.</ref>
+25<ref name="usp621">United States Pharmacopeia, General Chapter <621>, ''Chromatography''.</ref>
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21{{DEFAULTSORT:Limit of detection}}27{{DEFAULTSORT:Limit of detection}}