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Karl Fischer titration: difference between revisions

Diff·revision 35 → 36·14:47, 12 Feb 2025

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Revision 35 — 09:03, 3 Feb 2025
SigFigSindri (talk)
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Revision 36 — 14:47, 12 Feb 2025
AnalyticalAnnie (talk)
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61* '''Strongly bound water''' may not be released at cell temperature; an oven accessory that thermally desorbs water into the cell under dry carrier gas addresses this and is the usual arrangement for peptides with tightly bound hydration.61* '''Strongly bound water''' may not be released at cell temperature; an oven accessory that thermally desorbs water into the cell under dry carrier gas addresses this and is the usual arrangement for peptides with tightly bound hydration.
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+63Peptides containing free cysteine are the interference most relevant here, since a thiol-bearing sequence can give a falsely elevated water figure.{{cn}} A discrepancy between Karl Fischer and [[Loss on drying|loss-on-drying]] results on the same lot is a useful diagnostic: loss on drying measures all volatiles, so LOD substantially exceeding KF suggests [[Residual solvent|residual solvent]], whereas KF exceeding LOD suggests either an interference or water too tightly bound to be driven off at the drying temperature.{{r|usp1503}}
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63== References ==65== References ==
64{{reflist}}66{{reflist}}