Fmoc chemistry: difference between revisions
Diff·revision 2 → 3·19:54, 17 Jul 2024
Difference between revision 2 and revision 3 of Fmoc chemistry. 5 lines changed; the page grew by 436 bytes.
| Revision 2 — 00:48, 14 Jul 2024 DisparityDagny (talk) state that overlay gas is used and name it 1,514 bytes ±0 | Revision 3 — 19:54, 17 Jul 2024 ArchiveBot (talk) bot: normalise unit spacing 1,950 bytes +436 | ||
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| 11 | Its predecessor, Boc chemistry, used acid for both and therefore required a much stronger acid for final cleavage — hydrogen fluoride — with the handling requirements that implies. Boc chemistry was the scheme of the original solid-phase method.{{r|merrifield1963}} Orthogonality is what made peptide synthesis a routine operation.{{r|behrendt2016}} | 11 | Its predecessor, Boc chemistry, used acid for both and therefore required a much stronger acid for final cleavage — hydrogen fluoride — with the handling requirements that implies. Boc chemistry was the scheme of the original solid-phase method.{{r|merrifield1963}} Orthogonality is what made peptide synthesis a routine operation.{{r|behrendt2016}} |
| 12 | 12 | ||
| + | 13 | == The cycle == | |
| + | 14 | Fmoc is removed with a solution of piperidine in dimethylformamide, which abstracts the fluorenyl proton and triggers elimination. The released dibenzofulvene is trapped by excess piperidine to prevent it alkylating the peptide.{{r|behrendt2016}} | |
| + | 15 | ||
| + | 16 | The resin is then washed and the next Fmoc-protected amino acid coupled using an activating reagent. See [[Peptide coupling reagent]]. The cycle repeats once per residue. | |
| + | 17 | ||
| 13 | == References == | 18 | == References == |
| 14 | {{reflist}} | 19 | {{reflist}} |