Fmoc chemistry: difference between revisions
Diff·revision 14 → 15·05:45, 31 Dec 2024
Difference between revision 14 and revision 15 of Fmoc chemistry. 2 lines changed; the page grew by 206 bytes.
| Revision 14 — 12:07, 15 Dec 2024 HOMA_Hettie (talk) clarify that "cold chain" without a logger is a claim, not a record 3,512 bytes +124 | Revision 15 — 05:45, 31 Dec 2024 StopperingSteff (talk) add category for logistics 3,718 bytes +206 | ||
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| 27 | Racemisation occurs mainly at the activation step rather than at deprotection, and is worst at histidine and cysteine. It is invisible to [[Mass spectrometry|mass spectrometry]], since epimers are isobaric.{{r|behrendt2016}} | 27 | Racemisation occurs mainly at the activation step rather than at deprotection, and is worst at histidine and cysteine. It is invisible to [[Mass spectrometry|mass spectrometry]], since epimers are isobaric.{{r|behrendt2016}} |
| 28 | 28 | ||
| + | 29 | Diketopiperazine formation can cleave the first two residues from the resin when the third is being added, particularly with proline or glycine in those positions. Linker choice mitigates it.{{r|usp1503}} | |
| + | 30 | ||
| 29 | == References == | 31 | == References == |
| 30 | {{reflist}} | 32 | {{reflist}} |