Electrospray ionisation (revision 2)
Old revision·17:12, 5 Sep 2024·Chromatokid
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| Electrospray ionisation | |
|---|---|
| Abbreviation | ESI |
| Produces | Multiply charged ions from solution |
| Nature | Soft; molecule survives intact |
| Compatible with | Liquid chromatography |
| Analytical method infobox · conventions | |
Electrospray ionisation transfers molecules from solution into the gas phase as ions. A solution is sprayed from a capillary held at high potential, producing charged droplets whose solvent evaporates until ions are released.[1]
It is a soft technique — the molecule survives largely intact — and it produces multiply charged ions from peptides and proteins, so a large molecule appears at a mass-to-charge ratio within the range of ordinary analysers. A single peptide therefore produces a series of peaks at successive charge states, deconvolved by software into one neutral mass.[2]
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