Crude peptide: difference between revisions
Diff·revision 1 → 2·09:45, 19 Sep 2024
Difference between revision 1 and revision 2 of Crude peptide. 6 lines changed; the page grew by 782 bytes.
| Revision 1 — 10:37, 12 Sep 2024 CDMO_Caradoc (talk) new article: supply and handling, expansion welcome 1,088 bytes +1,088 | Revision 2 — 09:45, 19 Sep 2024 CustomsClarke (talk) rm the blanket claim that excursions are harmless 1,870 bytes +782 | ||
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| 10 | Its purity is highly variable and is largely determined by sequence. An easy 10-residue sequence may cleave at high purity; a difficult 40-residue sequence may cleave at well under half. That figure sets the cost and difficulty of everything that follows.{{r|behrendt2016}} | 10 | Its purity is highly variable and is largely determined by sequence. An easy 10-residue sequence may cleave at high purity; a difficult 40-residue sequence may cleave at well under half. That figure sets the cost and difficulty of everything that follows.{{r|behrendt2016}} |
| 11 | 11 | ||
| + | 12 | == What is in it == | |
| + | 13 | The dominant impurities are sequence-related: deletion sequences missing one or more residues where a coupling failed, truncated sequences where a chain was capped, and any peptide bearing residual protecting groups where cleavage was incomplete.{{r|behrendt2016,merrifield1963}} | |
| + | 14 | ||
| + | 15 | Cleavage adds its own. Strongly acidic conditions release reactive cations from the protecting groups, which can alkylate tryptophan, methionine and cysteine unless scavengers capture them; oxidation and, for susceptible sequences, aspartimide formation also occur. | |
| + | 16 | ||
| 12 | == References == | 17 | == References == |
| 13 | {{reflist}} | 18 | {{reflist}} |
| 14 | <ref name="behrendt2016">Behrendt R, White P, Offer J. "Advances in Fmoc solid-phase peptide synthesis." ''Journal of Peptide Science'' 22(1):4–27 (2016). PMID 26785684.</ref> | 19 | <ref name="behrendt2016">Behrendt R, White P, Offer J. "Advances in Fmoc solid-phase peptide synthesis." ''Journal of Peptide Science'' 22(1):4–27 (2016). PMID 26785684.</ref> |
| + | 20 | <ref name="merrifield1963">Merrifield RB. "Solid phase peptide synthesis. I. The synthesis of a tetrapeptide." ''Journal of the American Chemical Society'' 85(14):2149–2154 (1963). DOI:10.1021/ja00897a025.</ref> | |
| 15 | 21 | ||
| 16 | {{DEFAULTSORT:Crude peptide}} | 22 | {{DEFAULTSORT:Crude peptide}} |