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Bacteriostatic water (revision 4)

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Bacteriostatic water
CompositionWater for injection with 0.9% benzyl alcohol
Function of the preservativeInhibits microbial growth after entry
Contrast withSterile water for injection, unpreserved
Topic infobox · conventions

Bacteriostatic water is sterile water containing an antimicrobial preservative, conventionally benzyl alcohol at 0.9%. The preservative does not sterilise; it inhibits the growth of organisms introduced when a container is entered, which is what makes multiple withdrawals from one container possible. See Multi-dose vial.[1]

It is a diluent, not a treatment for non-sterile material. Adding it to a powder that was never sterile produces a non-sterile solution containing a preservative — the preservative retards growth of what is there rather than eliminating it.[2]

What the preservative does

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Antimicrobial effectiveness is a defined pharmacopoeial property: a preserved preparation is challenged with specified organisms and must reduce their numbers by defined amounts over defined intervals. Benzyl alcohol meets that requirement over the concentration and pH ranges in which it is used.[1]

Inhibition is not immediate and not universal. Some organisms are more resistant than the challenge panel, and preservative efficacy falls with dilution — a preserved diluent added to a large volume of unpreserved liquid may fall below its effective concentration.

The preservative also does nothing about chemical degradation. A preserved solution remains subject to deamidation, oxidation and aggregation, and the presence of a preservative does not extend chemical stability at all.[3]

References

  1. ^ a b United States Pharmacopeia, General Chapter <51>, Antimicrobial Effectiveness Testing.
  2. ^ United States Pharmacopeia, General Chapter <797>, Pharmaceutical Compounding — Sterile Preparations.
  3. ^ Manning MC, Chou DK, Murphy BM, Payne RW, Katayama DS. "Stability of protein pharmaceuticals: an update." Pharmaceutical Research 27(4):544–575 (2010). PMID 20143256.